Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 139)

Quantity One User Guide
5-22
The commands for contouring and drawing bands are located on the Contour
submenu and Draw Band submenu of the Band menu, and on the Contour Tools
toolbar.
Before using these commands, magnify the image so that the individual pixels in the
band are clearly visible. This allows you to position the cursor more accurately.
5.8.a Contouring Bands
Fig. 5-16. Contour tools.
Creating Contours
Select Create Contour from the Band > Contour submenu or toolbar and click on a
pixel at the edge of the band. This displays a contour that encloses pixels whose
intensity is equal to or greater than that of the pixel at the cursor.
Fig. 5-17. Creating a Contour
If the contour does not encircle the band, reposition the cursor and click again. A new
contour will be drawn in place of the old.
Contour encloses pixels
with intensities greater
than or equal to intensity
of pixel at cursor