Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 277)

Quantity One User Guide
C-4
C.2 Step I. Select Application
To set the appropriate parameters for the type of object you are imaging, click on the
Select button under Select Application. You have the option of selecting a UV, White,
or Chemiluminescence application. You can also select a custom setting. Once you
select an application, the name of the application and its settings appear in the Select
application step.
C.2.a. Custom Applications
The Bio-Rad installed applications have pre-set gain and bin settings. If the settings
for the available applications do not meet your needs, you have the option of creating
a custom application, which allows you to set your own gain and binning settings.
To use a custom application, click Select and choose custom. Next, select a custom
application from the list, or select Create to create a new application. This opens the
custom dialog.
Fig.3-3. Creating a custom ChemiDoc XRS application.
Enter a name for the application. From the Illumination drop down list, select an
illumination mode. You can select UV, white or no illumination. Next select gain and
binning settings from the drop down list.
A higher Binning setting (2x2, 3x3) provides optimal sensitivity for low-light
applications such as chemiluminescence. In this mode, the pixels in the camera are
“binned” (e.g., four pixels are combined into one) to increase the amount of signal per
pixel without increasing noise. Note that combining the pixels results in a reduction in
the resolution of the image.