Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 173)

Quantity One User Guide
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6.3.c Applying the Calibration Curve
After you have generated a calibration curve, you are ready to calculate the quantities
of the unknown bands. Select a button next to Calibrate to calculate the quantity of
an individual band, match, lane, or the entire gel.
Fig. 6-24. Buttons for calibrating bands of unknown quantity.
If you select Band, Match, or Lane, click on the button and then click on the object
in the gel. Click on Gel to calculate the entire gel. Bands of calculated quantity are
highlighted in the image.
Unapplying the Calibration Curve
To undo the quantity calculation for a band, match, lane, or gel, use the appropriate
button next to Un-Calibrate.
6.3.d Generating Standard Bands via a Dilution Series
One way to generate bands of known quantity is to start with a stock solution and
make several dilutions. Different dilutions can be loaded into different lanes, resulting
in a dilution series.
Quantity One will calculate the values in a dilution series if you enter the known
quantity and a dilution factor (e.g., for a solution that has been diluted to 10 times the
volume, type 1/10 or 0.1).
In the Quantity Standards dialog, next to the band from the undiluted stock solution,
enter the known quantity.
In the Dilution Factor column, type “stock.”
Next to the remaining bands in the series, enter the appropriate dilution factors in the
Dilution Factor column.