Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 124)

Chapter 5. Bands
5-7
Lane Width
The Lane Width setting determines the width along the lane lines that are sampled
for band detection and quantitation.
When a band is detected, an average intensity for each horizontal row of pixels within
the band brackets is calculated. The lane width determines the number of pixels in
each row. Study the band lines in the image while you adjust the width. Select a width
that is slightly wider than the bands in the gel.
Fig. 5-4. Adjusting the lane width.
You can also change the widths of individual lanes using the Lane Width command
(see section 4.1.d, Lane Width).
Minimum Density
When Normalize is turned off, the Min. Density defines the lowest signal intensity
that will be counted as a band.
Before selecting a Min. Density value, use the Plot Lane command on the Lane
menu to plot a trace of a lane that includes some faint bands. Then enter a value that is
lower than the intensity of the peak of a faint band but is still above the background.
If faint bands are still undetected after adjusting this parameter, you may want to
increase the Sensitivity setting.
Adjust the lane
width until the band
lines are slightly
wider than the bands
in the image.