Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 145)

Quantity One User Guide
6-2
Note: In general, the more standard lanes in a gel, the greater the accuracy of the
calculated band values. We recommend a minimum of two standard lanes per gel.
Also, the modeling algorithm works best with standard lanes spaced evenly
across the gel, as shown in the following figure. Multiple standard lanes also
facilitate comparisons of sample similarity.
Fig. 6-2. Standard lanes as defined on a gel image.
With the image open and lanes and bands defined, select Standards from the Match
menu or toolbar. A dialog will open, listing any saved standards as well as predefined
Bio-Rad standards.
Standard Lane Standard Lane Standard Lane