Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 186)

Chapter 7. Volume Tools
7-11
If you create more than one background volume, all the pixels in those background
volumes will be used to calculate the average background.
Background volume(s) will have default names B1, B2, etc., based on their creation
sequence. You can display/hide volume names using the Show/Hide Volume Labels
command.
Note: If the region you identified as background has a higher average intensity value
than a data object, that object will have a negative adjusted volume in the Volume
Analysis Report. If this happens, select a new background region that has less
intensity than the data object.
Displaying the Results of Background Subtraction
The Volume Analysis Report (see section 10.6, Volume Analysis Report) will display
both the unadjusted volume and the volume with background subtracted (adjusted
volume) of standards and unknowns, so you can see exactly how much intensity was
subtracted.
7.5 Volume Arrays
The Volume Array Tool on the Volume menu and toolbar can be used to quantitate
dot blots, slot blots, and other arrays.
Note: You cannot create a volume array in an image with asymmetric pixels (i.e.,
different dimensions in x and y). If you want to create a volume array in such an
image, select Reduce File Size from the File menu to change the pixel
dimensions of the image (see section 2.2.f, Reduce File Size).
What Is a Volume Array?
A volume array is a matrix of volume circles or rectangles that can be
sized/positioned as a group and overlaid on images of blots, wells, or cells for easy
quantitation. The individual cells in the array have the same functionality as standard
volumes. You can define cells as background volumes, standards, and/or unknowns,
as described in the sections above.