Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 315)

Quantity One User Guide
E-10
To set the automatic calibration settings, click on the More Options button in the GS-
710 acquisition window. This will open the Densitometer Options dialog box.
Fig. E-9. Densitometer Options dialog box.
E.5.a Editing the Step Tablet
Before you scan for the first time, you must enter the step tablet values for your
transmissive calibration strip into the Step Tablet Values form.
Note: In reflective scanning, the scanner is calibrated against the reflective step tablet.
However, you do not need to enter the values for this step tablet; you can use the
default values in the Step Tablet Values form.
Attached to the outside of your GS-710, you will find a copy of the manufacturer’s
printout of the diffuse density values for each segment of your transmissive step
tablet, as well as a serial number that identifies the step tablet to which those density
values belong. Those exact density values must be entered into the computer for the
software to associate a correct density value with each step on the step tablet.