Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 144)

6-1
6. Standards and Band
Matching
After you have defined the lanes and bands in a gel, you can identify the standard
lanes, enter the values of the standards, and determine the values of the experimental
bands using those standards. You can also compare sample similarity by matching
bands across lanes.
Finally, you can identify bands of known quantity and use these to generate a
calibration curve for quantitating unknown bands.
These tools are found on the Match menu and Match Tools toolbar.
Fig. 6-1. Standards and matching tools.
6.1 Standards
If your gel includes lanes of standards, you can enter the values of the standards and
automatically calculate the values of the unknown bands in the gel.