Bio-Rad Quantity One 1-D Analysis Software - Manual (Page 115)

Quantity One User Guide
4-18
The array matrix will appear on the image. Each column will be marked by a red line,
and each cell will be marked by top and bottom brackets.
Note: If the cells appear marked by lines instead of brackets, select Band Attributes
from the Band menu and select Brackets in the dialog.
When you first create the array matrix, it will probably not be centered on the
columns and cells in the actual image. In the next step, you will adjust the position of
the matrix.
Adjusting the Array Matrix
The Add/Adjust Anchors tool will be automatically assigned to the mouse after you
create the frame (otherwise, select it from the Lane > Edit Frame submenu). Position
the cursor on the corner points of the frame and drag them into position so that the red
lines run down the middle of the array columns and the top and bottom brackets are
centered on the array cells (see section 4.1.b, Editing the Frame for guidance on
adjusting frames).
If necessary, the Adjust Lane command (see “Adjusting Single Lanes” on page 7)
and Adjust Band command (see section 5.3.b, Adjusting Bands) can be used to
adjust the placement of columns and cells within the frame.
Reducing Background in the Array
After you have positioned the array, you should reduce lane background using the
Lane Background command (see page 4.2, Lane-Based Background Subtraction).
Lane background will affect quantitation of the cells.
Setting Array Cell Height and Width
Now you should adjust the cell brackets so that they completely enclose the cells in
the array.
Select Array Cell Height from the Lane > Lane-based Arrays submenu and enter the
height in millimeters of the array cells.