Bio-Rad BioLogic LP System - Manual (Page 11)

4
Introduction
Table 1. (continued)
Description of System Components
Component Function
System Rack This is the system organizer for columns and cartridges, buffer containers, sample inject
and buffer select valves, UV and Conductivity Flow Cells, and other devices used with the
system. The optional BioLogic Rack Expansion kit is available to extend the rack to 3
trays.
Proportioning This combination of valve and mixer module mixes and proportions two buffers.
Valve/Mixer It is designed to be rack mounted or free-standing.
Module
UV Detector This consists of the UV Optics Module and the control circuitry within the BioLogic LP
Controller. The UV Optics Module includes a mercury lamp and filters for fixed wave-
length UV detection at 280nm and 254nm. The flow cell has a path length of 2 mm, an
internal volume of 80
µ
l, and an illuminated volume of 3µl.
Conductivity This consists of the Conductivity Flow Cell and the control circuitry within the BioLogic LP
Detector Controller. The Conductivity Flow Cell provides instantaneous, on-line readings of con-
ductance for monitoring a salt gradient and optimization of column chromatographic condi-
tions.
Valves Valves for the BioLogic LP include:
• MV-6 Inject Valve
• SV-5 Buffer Select valve
• SV-3 Diverter/Column Bypass valves
Sample Loops Tygon tubing sample loops are prepared by the user.
Fraction Fraction collectors are controlled by the BioLogic LP Controller via the Fraction Collector
Collector port. Fraction advance marks (event marks) are embedded in the UV signal sent from the
Controller to a chart recorder. The following fraction collectors are supported:
• The Model 2128 provides X-Y motion drop dispensing. The Model 2128 accommodates a
wide range of tube diameters and lengths, microtiter plates, micro-tubes, and “bottle size”
fractions. The Model 2128 is ideally suited for both analytical and preparative applica-
tions.
• The Model 2110 uses a stationary drop-dispensing head to collect up to 80 fractions in a
carousel. The SV-3 Diverter valve is required for full operation. The Model 2110 is con-
trolled by the BioLogic LP Controller via the Fraction Collector port.
• Generic fraction collectors may be used with the BioLogic LP as long as tube advances
can be initiated by a TTL pulse (active high or active low, 100 ms duration.) The SV-3
Diverter valve is required for full operation.
Regardless of which fraction collector is chosen, fraction time/volume and collec-
tion parameters such as Collect All, Collection Windows, Threshold, and Threshold
+ Collection Windows are controlled from the BioLogic LP Controller.