Bio-Rad BioLogic LP System - Manual (Page 44)

37
System Operation
Table 6. (continued)
Manual Mode Operation: Pump
Reference Description
Calibration for standard tubing sizes (0.8 mm, 1.6 mm, 3.2
mm): If a standard tubing size is installed in the pump head,
you may select Nominal to use a nominal flow rate factor.
Selecting Set Flow allows you to do a “user calibration,” which
provides greater accuracy, as described below.
User Calibration (Set Flow) for improved flow accuracy:
This screen allows you to calibrate for standard tubing sizes by
experimentally determining the flow rate factor. This procedure
is advisable when using columns with high counter-pressure or
when improved flow rate accuracy is desired. Calibration
should be done with all components connected and the column
installed. To calibrate:
1.
Once the system is set up, select Purge to purge air from
the system. Select Stop to stop the pump, and place the
waste tube in a graduated cylinder.
2. Select Flow followed by Calibrate.
3. Select a standard tubing size, enter Flow Rate and Time,
and then press Start to calibrate. If more than one flow
rate is to be used, calibrate for the flow rate to be used dur-
ing the most critical period of the separation. For best
results, set a Time of at least 5 minutes. During calibration,
you can select Stop to shorten the calibration run.
4. At the end of the calibration period, when the pump has
stopped, enter the total volume delivered and press OK.
User Calibration for non-standard tubing size: To calibrate
for non-standard tubing (Other), follow the procedure above.
Calibration will proceed for the duration that you specify, at a
flow rate of 25% of full speed. Press Start to begin calibration.
Calibration complete: When the calibration run is complete,
enter the delivered volume and press OK. The pump is now
calibrated.
Method: <<name>> Manual Operation
Pump Calibration
Flow Rate: 1.00 ml/min Time: 5.00min
START FLOW RATE TIME CANCEL
Method: <<name>> Manual Operation
Pump Calibration
Flow Rate: 25% of Max Time: 0.00min
START TIME CANCEL
Method: <<name>> Manual Operation
Pump Calibration
Delivered Volume: 0.00
CANCEL OK
Method: <<name>> Manual Operation
Nominal Pump Calibration complete.
Calibrate pump at desired flow rate?
NOMINAL
SET FLOW CANCEL
M3b
M3c
M3c
M3a
M3b
M1 M1
M3e M1
Set flow rate and time
(duration of the calibration)
M3d M3a
M3e M1
Set time
M3e M3d M3c
M1