Bio-Rad BioLogic LP System User Manual

Page 2

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Table of Contents

TABLE OF CONTENTS

Safety
Getting Started Quickly

Chapter 1.0 Introduction...................................................................................................................1

1.1 Overview.........................................................................................................................1
1.2 Features .........................................................................................................................2
1.3 Unpacking.......................................................................................................................3
1.4 Description of System Components...............................................................................3

Chapter 2.0 Description of Components ........................................................................................6

2.1 BioLogic LP ....................................................................................................................6
2.2 Proportioning Valve/Mixer Module ..................................................................................9
2.3 UV Detector ....................................................................................................................10
2.4 Conductivity Detector .....................................................................................................11
2.5 Bio-Rad System Options ................................................................................................12

2.5.1 Model 2110 Fraction Collector ............................................................................12
2.5.2 Model 2128 Fraction Collector ............................................................................13
2.5.3 Model 1327 Chart Recorder................................................................................14

2.6 Non-Bio-Rad System Options ........................................................................................15

2.6.1 Non-Bio-Rad Fraction Collectors.........................................................................15
2.6.2 Non-Bio-Rad Chart Recorders ............................................................................16

Chapter 3.0 System Connections ....................................................................................................17

Chapter 4.0 System Plumbing .........................................................................................................22

4.1 General Guidelines for Plumbing the System ................................................................22
4.2 Adjusting the Platen and Plumbing The Peristaltic Pump ..............................................24
4.3 Plumbing the System .....................................................................................................26
4.4 Purging the System ........................................................................................................30

Chapter 5.0 System Operation.........................................................................................................31

5.1 Front Panel Controls.......................................................................................................31
5.2 Manual Mode Operation.................................................................................................34

5.2.1 Manual Mode Operation of the Pump .................................................................34
5.2.2 Manual Mode Operation of the Fraction Collector ..............................................38
5.2.3 Manual Mode Operation of the Alarms ...............................................................41
5.2.4 Manual Mode Operation of the UV Monitor ........................................................42
5.2.5 Manual Mode Operation of the Conductivity Monitor..........................................44
5.2.6 Manual Mode Operation of the Valves ................................................................46
5.2.7 Manual Mode Operation of the Chart Recorder..................................................48

5.3 Programming Mode ........................................................................................................49

5.3.1 Programming Mode’s Main Menu .......................................................................51
5.3.2 Creating a New Method ......................................................................................53
5.3.3 Viewing and Editing a Method ............................................................................56
5.3.4 Program Mode’s Pump Table ..............................................................................58
5.3.5 Program Mode’s Fraction Collector Table............................................................62

5.3.5.1

Collect All Mode ...................................................................................64

5.3.5.2

Threshold Collection Mode...................................................................65

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