Bio-Rad BioLogic LP System - Manual (Page 86)

Monitor Flow Cell and the Fraction Collector Drop Head. This is best determined empirically using a
syringe. To do this, first make certain the tubing path is full of fluid. Connect an empty syringe to the tubing
at the Fraction Collector Drop Head, disconnect the tubing from the UV Monitor Flow Cell, and then draw the
fluid from the path into the syringe. Measure the volume of the fluid in the syringe; this is the Delay Volume.
The Delay setting is remembered between runs because it is assumed that the fluid path does not change
between runs. Whenever the tubing is changed, the Delay setting should be redetermined unless the same
diameter and length of tubing is used to make the connections.
Note: When running time-based methods, where delay time is used instead of delay volume, the correct
delay setting must be determined using the formula below:
Delay Time = Delay Volume / Flow Rate
If multiple flow rates are used within the method, then calculate the delay using the flow rate when the most
critical peaks are expected to elute.
5.4.2 Run in Progress
While a run is in progress, the Run Screen is displayed. Runs do not require that the operator be present;
however there are a number of options available when present during the run. The information available
during a run is described in section 5.4.2.1. There are three types of action which can be taken by the oper-
ator during a run: Holding a Run, Pausing a Run, and Manual Override. These are discussed in sections
5.4.2.2 though 5.4.2.4 respectively. The Chart Recorder trace is discussed in section 5.4.3.
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System Operation
Figure 31. Run in Progress
Method: <<name>> Volume: 0.51 ml
Pump: 1.00 ml/min A UV: 0.0208 AU
Frac: No Program Cond: 0.10 mS
RUN STATUS HOLD PAUSE STOP RUN
CONTINUE
RESUME RUN
Are you sure;
multiple runs
will be cancelled:
yes/no
Run Vol: 0.51 ml End: 7.00 ml
Pump: Step 1 of 4 End: 1.00 ml
Frac: Next Window Start: 2.00 ml
RUN SCREEN
(return
to R2)
R2 RUN SCREEN
RUN STATUS SCREEN
HOLD CONDITION
R2a
PAUSE CONDITION R2c
R2b
R2
R2a
R2b
R2c
LEGEND