Bio-Rad BioLogic LP System - Manual (Page 17)

10
Description of Components
2.3 UV DETECTOR
The UV Detector, which consists of the UV Optics Module and the control circuitry within the BioLogic LP
Controller, is a single beam, fixed wavelength UV absorbance detector specifically designed for protein chro-
matography. The UV Optics Module can be positioned close to a column outlet, minimizing system dead
volume and remixing of peaks. When connecting the UV Optics Module to the system, note the flow direc-
tion arrows on the top of the UV Optics Module’s case. It is important that the flow direction through the flow
cell is correct.
Figure 3. UV Optics Module
The AU readings from the BioLogic LP UV detector will not correspond directly to absorbance readings from
a spectrophotometer. The primary reason for this is the different path lengths; light bandwidths and other
factors have an effect as well. This has no effect on the UV monitor’s ability to detect protein.
Eleven fixed UV absorbance ranges, from 5.0 to .001 Absorbance Units Full Scale (AUFS), are available.
The user may choose a “custom” range using the “Set Max” function. Refer to Table 9.
The UV Optics Module consists of a low pressure mercury lamp, 280nm and 254nm filters, and a flow cell.
The filters are both held by a single tray. The flow cell, which uses luer fittings, has a 2 mm path length. It
has an internal volume of 80
µ
l and an illuminated volume of 3 µl.
The UV Optics Module receives power from the BioLogic LP Controller, to which it is connected by the UV
Lamp cable. The UV detector communicates with the system via the UV Optics cable, which plugs into the
UV Optics connector on the back of the BioLogic LP Controller.
FILTER HOLDER
AND FILTERS
FLOW CELL
UNDERSIDE OF
UV OPTICS MODULE