Bio-Rad BioLogic LP System - Manual (Page 56)

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System Operation
5.3 PROGRAMMING MODE
Programming mode is used to define a sequence of actions which collectively comprise a method. Once a
method is written the BioLogic LP can carry out the programmed actions unattended by running the method.
To enter the Programming Mode press the Program mode key. When viewing a method, the “Method List”
presents all programmed actions in the order in which they will occur when the method is run. The Method
List is generated automatically each time a method is created, modified, or recalled from storage (opened).
The Method List cannot be created or modified directly. The process of creating or modifying a method con-
sists of completing three separate tables which together comprise the method:
1. Pump Step Table: This table is used to define all pump parameters throughout the entire method.
The table is divided into numbered steps and can contain up to 50 steps. Each step includes a
buffer, a duration or length, and a flow rate. The buffer can be a single solution, a mixture of two
solutions, or a linear gradient. The duration of a step will be in either units of time (minutes) or vol-
ume (ml) depending upon the choice made when the method is created. The flow rate will be in
ml/min. To view or make changes to the Pump Step Table press the Pump instrument key.
2. Fraction Collector Table: This table is used to define the fraction collection parameters for the
entire method. Fraction collection can be programmed in any of four different ways including collect-
ing the entire run (“Collect All”), collecting only when the UV detector signal indicates a peak
(“Threshold”), collecting during specific periods in the run only (“Windows”), and a combination of
the threshold and windows schemes (“Threshold + Windows”). Collection parameters such as frac-
tion size and collection “windows” will be programmed in units of time (minutes) or volume (ml)
depending upon the choice made when the method is created. To view or make changes to the
Fraction Collection Table press the Collector instrument key.
Note: Fraction collection is not required in a method although most methods will typically include
fraction collection. When Windows are used the method may contain up to 50 windows.
3. Alarm Table: This table is used to define up to three specific points in the progress of a run when
an alarm will sound. If desired, a Hold can be placed at the time of an alarm to stop the progress of
the run until the user chooses to continue the run. To view or make changes to the Alarm Table
press the Alarm instrument key.
Note: Alarms are not required in a method and are only valuable when the user will be nearby dur-
ing the run.
Figure 21 shows the relationship between the contents of the three tables described above and the Method
List.