Bio-Rad BioLogic LP System - Manual (Page 36)

9. Plumbing the UV Optics Module and Conductivity Flow Cells.
a. Connect the column outlet line to the inlet port of the UV Optics Module.
(Arrows on the UV Optics Module indicate flow direction.)
b. Cut a short length of Tygon tubing and attach luer fittings to each end.
Screw one end into the output port of the UV Optics Module’s flow-cell and the other end into the
Conductivity Flow Cell. (Note: The Conductivity Flow Cell is bi-directional.)
c. Place the Conductivity flow cell into its holder.
10. Connection to a Model 2110 Fraction Collector or Non-Bio-Rad Fraction Collector. Note: This
step involves the use of an SV-3 Diverter valve.
a. Connect a piece of Tygon tubing between the Conductivity flow cell and the SV-3 Diverter valve
common port (labeled “Common”) using the luer fittings.
b. Connect a piece of Tygon tubing from the SV-3 Diverter valve’s “Waste” port for use as a waste line.
c. Connect a piece of Tygon tubing from the SV-3 Diverter valve’s “Collect” port to the Model 2110 frac-
tion collector drop-head. This tubing sits inside the drop-head, and no fittings are required.
11. Connection to a Model 2128 Fraction Collector.
For further discussion on plumbing the Model 2128, refer to the Model 2128 Fraction Collector
Instruction Manual.
Note: The tubing’s length must allow unrestricted movement of the fraction collector arm.
When using the Model 2128 Fraction Collector with the BioLogic LP, you have three options for diverting
“waste” fluid.
a. No diverter valve used: In this configuration, tubing from the Conductivity flow cell is connected
directly to the fraction collector’s drop head. This requires a female luer adapter (supplied with the
Model 2128) to connect the tubing from the Conductivity flow cell to the 1/16” tubing from the Model
2128. This will require using a barb-to-male fitting from the BioLogic LP fittings kit. (Note: Be sure
that System Cable 3 is connected between the Model 2128 and the Controller’s Diverter Valve port.)
When you turn on the Model 2128, select “Econo” mode from the Model 2128’s startup screen. The
system prompts with the message “Is 2128 valve cable connected.” (This refers to System Cable
3.) Answer YES.
b. Using the Model 2128’s arm-mounted diverter valve: Tubing from the Conductivity flow cell is
connected to the diverter valve’s Common port. This requires a female luer adapter (supplied with
the Model 2128) to connect the tubing from the Conductivity flow cell to the 1/16” tubing from the
Model 2128. This will require using a barb-to-male fitting from the BioLogic LP fittings kit.
Plumb the diverter valve’s Collect port to the drop head and its Waste port to a suitable waste con-
tainer. Use the 1/16” tubing and ferrules supplied with the Model 2128.
(Note: Be sure that System Cable 3 is connected between the Model 2128 and the Controller’s
Diverter Valve port.) When you turn on the Model 2128, select “Econo” mode from the Model
2128’s startup screen. The system prompts with the message “Is 2128 valve cable connected.”
(This refers to System Cable 3.) Answer YES.
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System Plumbing