Bio-Rad BioLogic LP System User Manual
Page 93
6.2.1 Nominal Calibration
Nominal flow rate factors for the three standard tubing sizes (0.8, 1.6, and 3.2mm ID) are programmed into
the system at the factory. Many users find that the flow rate accuracy obtained with “nominal” calibration is
sufficient for their purposes. To use the nominal calibration values:
1.
At the BioLogic LP’s front panel, press the Manual mode button, followed by the Pump instrument
button. This will display the pump’s Manual mode screen.
2.
Press the Flow softkey.
3.
Press the Calibrate softkey. From the three tubing size options, press the softkey for the tubing
size you will use.
4.
Press the Nominal softkey. The system then will use the nominal calibration for the tubing size you
have selected.
6.2.2 User Calibration
The user calibration feature allows the user to determine a flow rate factor experimentally and to program
this factor into the system. This procedure is advisable when using columns with high counterpressure,
when using pump head tubing of a non-standard size, or whenever precise flow rate accuracy is desired.
For best accuracy, calibrate the system with your column installed, and all other system components con-
nected. Set the calibration flow rate to the flow rate you will be using during your run. If multiple flow rates
will be used, calibrate at the flow rate for the most critical part of the separation.
To perform a user calibration,
1.
Connect your system as you intend to use it, including the column. Place the inlet tubes in contain-
ers of buffer. Make sure the “waste” tube from the system also will reach a graduated cylinder
placed on the bench.
2.
Press the Manual mode button, followed by the Pump instrument button to display the Pump’s main
Manual mode screen.
3.
With the “waste” tube in a waste container, press the Purge softkey to purge air from the system.
4.
When the purge is complete, from the pump’s main Manual mode screen, press the Flow softkey.
5.
Press the Calibrate softkey to display the three tubing size options. Press the softkey for the tubing
size you will be using. If you are using a non-standard tubing size in the pump head, press the
Other softkey and proceed to step 7; the system will calibrate at 25% of full pump speed.
6.
Press the Set Flow softkey, followed by the Flow Rate softkey. Using the numeric keypad, enter
the flow rate for the calibration. Press the OK softkey.
7.
Press the Time softkey. Using the numeric keypad, enter the total time period for the calibration.
(For best results, enter a time of 5 minutes.) Press the OK softkey.
8.
Place the “waste” outlet tube into the empty graduated cylinder. Press the Start softkey. The pump
will run and the display will count down the time remaining.
9.
At the end of the calibration period, the pump will stop. Using the numeric keypad, enter the total
volume delivered to the graduated cylinder. Press the OK softkey. The system is now calibrated for
the tubing and system configuration used.
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Maintenance and Troubleshooting