Bio-Rad BioLogic LP System - Manual (Page 66)

Table 16. (continued)
Program Mode’s Pump Table
Reference Description
Select Buffer: The buffers available are indicated by the letters
A through E plus the letters G and M which are shown on line 2
of the LCD. If a buffer is available the letter will be an upper
case letter. When a buffer is not available it is shown in a lower
case letter.
The availability of buffers is controlled by the presence or
absence of the system valves. Buffer A is always available.
Buffer B becomes available when the Proportioning Valve/Mixer
is plugged into the Controller. Buffer C becomes available
when an SV3-2 valve is plugged into the Buffer Selector port.
Buffers C, D, and E become available when an SV5-4 valve is
plugged into the Buffer Selector port. The Controller senses the
presence of the valves and automatically updates this list as
valves are plugged and unplugged from the Controller.
For convenience, all buffers can be programmed in a method
even when not currently available. To run the method the
appropriate valves must be connected.
The letters G and M stand for Gradient and Mixture. Gradient
refers to a linear gradient made from buffers A and B and
Mixture refers to a single solution made by mixing buffers A and
B. Gradient and Mixture compositions are expressed in %B.
The brackets around one of the buffers, [A], indicate which
buffer is currently selected. Use the Prev and Next keys to
change the selection.
• Grad: This is used to program a linear gradient in this step.
• Mix: This is used to program a mixture in this step.
• Cancel: This cancels the addition or modification of this
step and returns to the Pump Table as it was originally.
• OK: This confirms the selected buffer as indicated by the
brackets surrounding the letter and continues to the next
entry.
Note: The Grad and Mix softkeys are identical to selecting
the G and the M respectively from the list.
59
System Operation
Method: Volume: 1.00 ml
Step: 1 Buffers: G M [A] B C D E
(GRAD=Linear Gradient, MIX=%B Mixture)
GRAD MIX CANCEL OK
P2
P2a P2b P3
(return
to P1)