4 manual mode operation of the uv monitor – Bio-Rad BioLogic LP System User Manual

Page 49

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5.2.4 Manual Mode Operation of the UV Monitor

Table 9 discusses each of the functions available in Manual mode operation of the UV Monitor. For an
overview of these functions, refer to Figure 18.

Table 9.

Manual Mode Operation: UV Monitor

Reference

Description

To manually control the UV Monitor:
a.

From the Mode keys select Manual.

b.

From the Instrument keys select UV.

c.

The main menu is shown below.

The display shows the following:

UV Monitor: The current UV reading.

Range/Max Range: The chart range for UV detection.

Description of the soft key choices follows.

Set Range: Allows you to increase/decrease the full-scale AU
(Absorbance Unit) value for the UV output to the chart recorder.
For example, when the range is set to 1.000 AUFS
(Absorbance Units Full Scale), the chart recorder pen will be at
its highest point (“full scale”) when the absorbance of the sam-
ple in the flow cell is 1 AU. The following set ranges are avail-
able: 2.0, 1.0, 0.5, 0.2, 0.1, 0.05, 0.02, 0.01, 0.005, 0.002,
0.001 AUFS.

Zero: This allows you to zero the UV baseline on the chart
recorder.

42

System Operation

Method: <<name>>

Manual Operation

UV Monitor: 0.0111 AU

Range: 1.000 AUFS

SET RANGE

ZERO

SET MAX

LAMP

MU

M3

M4

M1

M2

Method: <<name>>

Manual Operation

UV Monitor: 0.0111 AU

Range: 1.000 AUFS

SET RANGE

ZERO

SET MAX

LAMP

M2

M3

M4

M1

Method: <<name>>

Manual Operation

UV Monitor: 0.0111 AU

Range: 1.000 AUFS

INCREASE

DECREASE

CANCEL

OK

M1

MU

MU

(Increment/decrement

the range by

approximately 50%

each time the button

is pressed)

(sets the

current UV

reading to

0.0000)

Manual

UV

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