Bio-Rad BioLogic LP System - Manual (Page 60)

5.3.2 Creating a New Method
To create a new method enter Programming mode by pressing the Program mode key. From the
Programming mode main menu the New Method softkey is used to create an entirely new method. Note: It
is often easier to open a stored method which is similar to the desired new method, modify it as desired, and
then save it using a new name. See section 5.3.3 for a description of how to do this.
Table 14.
Program Mode Operation: New Method
Reference Description
Select Time or Volume mode: The units used to define the
actions within a method can be either time (minutes) or volume
(ml). Once this selection is made the method cannot be
changed from one unit type to the other unit type. After this
selection is made the method actions are programmed starting
with the pump steps. For a description of programming pump
steps see section 5.3.4. When all pump steps have been pro-
grammed, the OK softkey displays the Method List containing
only pump steps.
Method List: This list shows the actions which will take place
when the method is run in the order in which they will occur.
The numbered steps indicate the pump steps within the
method. Use the Prev and Next keys to scroll through the list
and view the method. The units of duration are in either time
(minutes) or volume (ml) depending upon the choice made
when the method was created.
If additional pump steps or changes to the pump steps are
desired see section 5.3.4. If fraction collection is desired during
this method see section 5.3.5 which describes fraction collec-
tion programming. If Alarms are desired within the method see
section 5.3.6 which describes alarm programming.
After each table is completed the Method List will be displayed
again including the changes made and can be reviewed . You
will notice that the list shows fraction collection and alarms as
events and places them directly under the pump step in which
they occur. When all desired actions have been programmed
and the method is complete, press the Done softkey.
Notes: A method must contain at least one pump step; however
fraction collection and alarms are optional. The other BioLogic
LP instruments (UV Monitor, Conductivity Monitor, Chart
Recorder, and Valve Controller) are not part of programmed
methods. They can be operated by pressing the
53
System Operation
Method: <<untitled>> Volume: 0.00 ml
Select Programming Mode
TIME VOLUME CANCEL
PN
N1
(return to PM)
List: Volume(ml) Buffer ml/min
01 0.00 to 1.00 0-1%B 1.00
02 0.00 to 2.00 1-10%B 1.00
PUMP FRAC COLL ALARM DONE
L1
Refer to Sections 5.3.4 - 5.3.6