Table 16. (continued) program mode’s pump table – Bio-Rad BioLogic LP System User Manual
Page 67
Table 16. (continued)
Program Mode’s Pump Table
Reference
Description
Linear Gradient: A linear gradient consists of a continuous
change in the buffer composition from an initial mixture of
buffers A and B to a final mixture of buffers A and B. Buffer
composition is set in %B such that %A + %B = 100%.
•
Initial: This places the cursor in the field to enter buffer
composition at the start of the gradient. Enter the value
using the number keys.
•
Final: This places the cursor in the field to enter buffer com-
position at the end of the gradient. Enter the value using
the number keys.
•
Cancel: This cancels the addition or modification of this
step and returns to the Pump Table as it was originally.
•
OK: This confirms the linear gradient entered and continues
to the step duration entry.
Note: The Prev and Next keys also move the cursor
between the initial and final values.
Mixture: A mixture continuously delivers a combination of
buffers A and B. This delivery is isocratic or constant through-
out the step. The buffer composition is set in %B such that
%A + %B = 100%. Enter the value using the number keys.
•
Cancel: This cancels the addition or modification of this
step and returns to the Pump Table as it was originally.
•
OK: This confirms the isocratic mixture entered and contin-
ues to the step duration entry.
Length of Step: The duration of this step is entered using the
number keys. The duration is in either units of time(minutes) or
volume (ml) depending upon the choice made when the method
was created.
•
Cancel: This cancels the addition or modification of this
step and returns to the Pump Table as it was originally.
•
OK: This confirms the length of step entered and continues
to the step flow rate entry.
Flow Rate: The flow rate of this step is entered using the num-
ber keys. The flow rate entry is the final entry for a pump step.
•
Cancel: This cancels the addition or modification of this
step and returns to the Pump Table as it was originally.
•
OK: This confirms the flow rate entered thereby completing
the step and returns to the Pump Table with the new or
modified step included in the table.
60
System Operation
Method:
Volume: 1.00 ml
Step: 1 Linear Gradient Conditions
Initial: 0%B
Final: 0%B
(initial)
FINAL
CANCEL
OK
P2a
P3
(return
to P1)
(set the ini-
tial value)
(set the
final value)
Method:
Volume: 1.00 ml
Step: 1
Isocratic Mixture Conditions
Mixture: _ %B
CANCEL
OK
P2b
P3
(return
to P1)
Method:
Volume: 1.00 ml
Step: 1
Length of Step: 0.00 ml
CANCEL
OK
Method:
Volume: 1.00 ml
Step: 1
Flow Rate: 1.00 ml/min
CANCEL
OK
P3
P4
(return
to P1)
P4
(return
to PT)