Bio-Rad BioLogic LP System - Manual (Page 42)

35
System Operation
Method: <<name>> Manual Operation
Flow: 1.00 ml/min UV: 0.0111 AU
Buffer: A Cond: 0.000 mS
START PURGE FLOW BUFFER
Method: <<name>> Manual Operation
Flow: 1.00 ml/min UV: 0.0111 AU
Buffer: A Cond: 0.000 mS
STOP RUN BUFFER
STOP
pump continues
at run rate pump
stops
Method: <<name>> Manual Operation
Buffers: M [A] B C D E
(Select MIX for Mixture of A and B)
MIX
CANCEL OK
Method: <<name>> Manual Operation
Enter Mixture: 50%B
(Mixture will contain buffers A and B)
CANCEL OK
(return to MP)
(If [A-E], go to
M1screen)
(If [M], go to
next screen)
(return to MP)
(return to MP)
(return to MP)
(return to MP)
(return to MP)
(return to MP) Set Flow Rate or Time,
press OK
and return to this screen
Set Time,
press OK
and return
to this screen
Method: <<name>> Manual Operation
Flow: 1.00 ml/min UV: 0.0111 AU
Buffer: A Cond: 0.000 mS
CALIBRATE FORWARD CANCEL OK
REVERSE
Method: <<name>> Manual Operation
Current Tubing Diameter: 3.2 mm
Press "PUMP" to CANCEL
0.8 mm 1.6 mm 3.2 mm OTHER
Method: <<name>> Manual Operation
Pump Calibration
Flow Rate: 25% of Max Time: 0.00min
START TIME CANCEL
Method: <<name>> Manual Operation
Pump Calibration
Flow Rate: 1.00 ml/min Time: 5.00min
START FLOW RATE TIME CANCEL
Method: <<name>> Manual Operation
Pump Calibration
Delivered Volume: 0.00
CANCEL OK
Method: <<name>> Manual Operation
Nominal Pump Calibration complete.
Calibrate pump at desired flow rate?
NOMINAL SET FLOW CANCEL
MP
M3
M3a
M3b
M3c
M3d
MP MANUAL MODE: PUMP
PUMP PURGE MENU
PUMP FLOW MENU
FLOW CALIBRATE MENU
CALIBRATE FOR PRESET TUBING SIZE
SET FLOW FOR PRESET TUBING
SET TIME FOR OTHER TUBING SIZE
CALIBRATION COMPLETE
BUFFER MENU
ENTER MIXTURE
M2
M3
M3a
M3b
M3c
M3d
M3e
M4
M4a
M3e
M2 M1
M3d
M4
M4a
LEGEND
Figure 15. Manual Operation of the Pump
(Screen displays are keyed to the discussion in Table 6, Manual Mode Operation : Pump)