Bio-Rad BioLogic LP System - Manual (Page 45)

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System Operation
Table 6.
Manual Mode Operation: Pump
Reference Description
Buffer screen: Selecting Buffer from the Main Menu screen
displays the Buffer screen, shown to the left. This screen
allows you to select the buffer to be delivered to the column.
Use the Prev and Next keys to select a single buffer (A through
E) or a mixture of buffers A and B. To set a mixture, select the
Mix soft key and enter the percent of buffer B. Note: If a valve
is not connected on the BioLogic LP, its letter or letters appear
in lower case and cannot be selected.
5.2.2 Manual Mode Operation of the Fraction Collector
Table 7 discusses each of the functions available in Manual mode operation of the fraction collector. An
overview of the Fraction Collector’s Manual mode operation is shown in Figure 16.
Table 7.
Manual Mode Operation: Fraction Collector
Reference Description
To begin Manual mode operation of the fraction collector:
a.
From the Mode keys select Manual.
b. From the Instrument keys select Collector.
c. The main menu for manual mode operation of the fraction
collector is shown below.
The display shows the following:
•
Fraction Size: The fraction size can be specified as a vol-
ume (ml) or time (minutes).
• UV: The current UV reading.
• Diverting to Waste/ Collect: Indicates the position of the
SV-3 Diverter valve.
• Cond: The current Conductivity reading.
Description of the soft key choices follows.
Method: <<name>> Manual Operation
Buffers: M [A] B C D E
(Select MIX for Mixture of A and B)
MIX CANCEL OK
M4
M4a
M3
M1
Method: <<name>> Manual Operation
Fx Size: 1.00 ml UV: 0.0111 AU
Diverting to Waste Cond: 0.000 mS
START F/X SIZE ADVANCE MODEL
MF
M3 M1 M2 M4
Manual Collector