Bio-Rad BioLogic LP System User Manual
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c.
Using an SV-3 Diverter valve: Mount the valve on the System Rack so that it is as close as possi-
ble to the Model 2128 Fraction Collector. Plumb the system as shown in Figure 14. (Note: This
configuration requires the valve be connected to the Diverter Valve port on the BioLogic LP.)
When you turn on the Model 2128, select “Econo” mode from the Model 2128’s startup screen. The
system prompts with the message “Is 2128 valve cable connected.” (This refers to System Cable
3.) Answer NO.
Note: If you answer YES, no fractions will be collected because there will be no System Cable 3 to
send commands from the BioLogic LP. To correct the problem, you will need to use the fraction col-
lector’s control panel to “park” the drop head and arm and then de-select “Econo” mode. Then re-
select “Econo” mode and answer NO to the message “Is 2128 valve cable connected.”
4.4 PURGING THE SYSTEM
Before a method can be run, you must take proper steps to evacuate all air from the system.
1.
Make sure the column is not connected to the system and the column inlet tubing is connected to the
column outlet tubing.
2.
Verify that all buffer inlet lines are submerged in a container of high quality water or buffer.
3.
Turn on the BioLogic LP Controller. When you turn on the BioLogic LP Controller, the system automati-
cally shows Manual mode for Pump operation. Verify that the arrow on the left side of line 2 of the LCD
points to the right.*
4.
Press the Purge softkey to run the pump at its maximum flow rate. Water or buffer will now flow
through the system.
5.
While the pump is running, press the Buffer softkey and cycle through all of the ports on the SV-5 Buffer
Select valve. After all buffer lines have been filled with liquid, stop the pump.
6.
Using the system’s manual mode operation of the pump, set a flow rate that is safe for the column. To
do this,
a.
From the front panel of the BioLogic LP, press the Manual key, followed by the Pump key.
b.
Using the softkeys below the front panel’s display, press the Flow softkey.
c.
Using the front panel’s keypad, enter a flow rate that is safe for the column.
7.
Disconnect the column inlet and column outlet lines and insert the column. Avoid introducing air to the
inlet side of the column.
8.
Press the front panel’s Run key to purge air which may have entered the system when the column was
connected.
*This arrow indicates the direction of flow. If the arrow points to the left press the Flow softkey followed
by the Forward softkey to change the direction of flow. Finally press the OK softkey to confirm the
change.
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System Plumbing