Bio-Rad BioLogic LP System User Manual
Page 33
4.3 PLUMBING THE SYSTEM
1.
Plumbing the inlets to the Proportioning valve/Mixer module.
a.
Cut two suitable lengths of Tygon tubing (0.8mm ID or 1.6mm ID).
b.
Attach the barbed end of a male luer to one end of each piece of tubing. (Warming the Tygon tub-
ing in warm water makes it more flexible and easier to work with.)
c.
Connect each fitting to one inlet port of the Proportioning valve. Twist the fitting clockwise to lock.
Do not over-tighten.
d.
Immerse the ends of the tubing in a container of high quality water or buffer.
2.
Plumbing the outlet from the Proportioning valve/Mixer module.
If you are not using the optional SV-5 Buffer Select valve, plumb the Mixer module’s outlet to the left
port of the pump. Then proceed to step 5.
If you are plumbing to the optional Buffer Select Valve:
a.
Cut a suitable length of Tygon tubing that will reach from the Proportioning valve/Mixer module outlet
to the SV-5 Buffer Select valve.
b.
Attach male luer fittings to each end.
c.
Connect the tubing to the outlet port on the Proportioning valve/Mixer module and to port A/B of the
SV-5 Buffer Select valve. Twist clockwise to lock. Do not over-tighten.
3.
Plumbing the outlet from the SV-5 Buffer Select Valve to the BioLogic LP Controller’s pump.
a.
Cut a length of Tygon tubing that will reach from the SV-5 Buffer Select valve to the BioLogic LP
Controller’s pump.
b.
Attach male luer fittings to each end.
c.
Connect the fitting to the Common port on the SV-5 Buffer Select valve and to the left port of the
pump. Twist clockwise to lock. Do not over-tighten.
4.
Plumbing from Buffer Reservoirs C through E to the Buffer Select Valve.
a.
Cut a length of Tygon tubing that will reach from each Buffer Reservoir to the SV-5 Buffer Select
valve.
b.
Attach the male luer to one end of each piece of tubing.
c.
Screw the tubing into inlet ports C through E of the SV-5 Buffer Select valve. Twist clockwise to
lock. Do not over-tighten.
d.
Immerse the ends of the tubing in a container of high quality water or buffer.
5.
Plumbing from the BioLogic LP Controller’s pump to the MV-6 Inject Valve.
a.
Use the screw fitting on the MV-6 Inject valve to secure the valve to the system rack. Turn the MV-6
knob counterclockwise so it matches that shown in Figure 14.
b.
Cut a suitable length of Tygon tubing that will reach from the BioLogic LP Controller’s pump to the
MV-6 Inject valve.
c.
Attach male luer fittings to each end.
d.
Connect the tubing to the pump’s right port and to the MV-6 Inject valve and secure firmly using a
twisting action. Turn clockwise to lock; do not overtighten.
26
System Plumbing