Bio-Rad BioLogic LP System - Manual (Page 69)

5.3.5 Program Mode’s Fraction Collector Table
The Fraction Collector Table displays the fraction collector parameters within the method. This table is
viewed upon the completion of selecting a collection mode and entering the necessary parameters. To
begin this process press the Collector instrument key while in Program mode or press the Frac Coll softkey
on the Method List screen. If fraction collection has not been programmed within the method, the Select
Collection Mode screen will be presented. There are four different fraction collection modes which can be
used: Collect All, Threshold Collection, Collection Windows, and Threshold plus Collection Windows. Each
of these modes will be discussed individually below. If a fraction collection mode was previously pro-
grammed within the method, then the Fraction Collection Summary screen will be presented.
Table 17.
Program Mode’s Fraction Collector Table
Reference Description
Select Collection Mode: The fraction collection mode is cho-
sen using the softkeys. There must be an SV3-2 valve (or
System Cable 3 with a Model 2128) plugged into the Diverter
Valve port on the Controller to run a method using any collec-
tion mode except Collect All.* After making a selection, the
parameter entry screen(s) for that collection mode will be pre-
sented. For descriptions of these screens consult the appropri-
ate sections below.
Note: If no fraction collection is desired use the Pump key or
Alarm key to leave this screen without making a selection.
•
All: Selects Collect All mode which collects fractions
throughout the entire method. See section 5.3.5.1.
• Threshold: Selects Threshold Collection mode which
allows you to only collect peaks by defining a threshold
value in absorbance units (AU). See section 5.3.5.2.
• Windows: Selects Collection Windows mode allows you to
specify periods called “windows” within the method when
fractions will be collected. See section 5.3.5.3.
• Thresh+Win: Selects Threshold plus Collection Windows
mode is a combination of the two modes described above.
See section 5.3.5.4.
*For convenience, all collection modes can be programmed
without the valve connected.
62
System Operation
Method: Volume: 1.00 ml
Select a Fraction Collector Mode
ALL THRESHOLD WINDOWS THRES+WIN
F1
F2 F3 F4 F5