Bio-Rad BioLogic LP System User Manual
Page 35
6.
Plumbing the MV-6 Valve’s Sample Loop.
Plumb the inject valve’s sample loop according to Figure 14. Use male luer fittings at each end of the
loop tubing. A sample loop of any volume can be prepared. Volumes may be determined empirically, or
by following the example below:
Total volume of sample = Valve volume + Fittings volume + Tubing volume
where,
Volume valve passage and fittings = .325 ml
Tubing volume = Length of tubing x Volume/cm, as determined from the following:
ID
Volume/cm
0.8
.005 ml/cm
1.6
.020 ml/cm
3.2
.08 ml/cm
Example: A 2 ml sample loop using 1.6 mm ID tubing:
a.
Subtract volume of fittings and valve passage:
2 ml - 0.325 ml = 1.675 ml
b.
Divide the tubing volume needed by the volume/cm of tubing:
1.675 ml ÷ 0.020 ml/cm = 83.75 cm of tubing
In this example, the volume of the valve and fittings, plus the internal volume of 83.75 cm of the 1.6 mm
ID tubing, equals 2 ml.
7.
Plumbing the SV-3 Bypass valve. (Optional)
Note: If you will not be using the optional SV-3 Bypass valve, proceed to the next step.
a.
Connect a length of Tygon tubing between the MV-6 Inject valve and the SV-3 Bypass valve com-
mon port (labeled “Common”) using male luer fittings.
b.
Connect a piece of Tygon tubing from the ‘Bypass” port of the SV-3 Bypass valve (see figure 14) to
a suitable waste container.
8.
Plumbing the Column.
Note: The column will be positioned in the fluid path
after the system is purged of air. In its place, tem-
porarily connect the column inlet line to the column outlet line.
a.
Plumb the column inlet as follows:
•
If you are using an SV-3 Bypass valve, cut a length Tygon tubing that will reach between the
SV-3 Bypass valve and the column inlet. Attach male luer fittings to each end. Connect one
end to the SV-3 valve’s “Collect/Column” port.
•
If you are not using the SV-3 Bypass valve, cut a length that will reach between the MV-6 Inject
valve and the column inlet. Attach male luer fittings to each end. Connect one end to the MV-6
valve’s “Outlet” port.
b.
To plumb the column outlet, cut a length of tygon tubing that will reach from the column outlet to the
UV Optics Module. Attach a male luer fitting at one end and a female luer fitting at the other.
c.
Connect the column inlet line to the column outlet line. This will be disconnected to insert the col-
umn after purging the system.
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System Plumbing