2 time course scan reading, 3 analysis of time course data – Hach-Lange DR 6000 User Manual User Manual

Page 114

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Expanded programs

6.6.2

Time course scan reading

After all scan parameters have been selected, the instrument
must then be zeroed. Afterward the sample can be analyzed.

1. Insert the zero solution cell in the cell compartment and

close the cell compartment cover.

2. Press Zero.

The zero value is shown in the display.

3. Insert the analysis cell in the cell compartment and close the

cell compartment cover.

4. Press Read.

The instrument will begin collecting Time Course data (kinetic
data).

Note: During the reading the Zero and Read keys change to Mark
and Stop.

Select Mark to mark the next data point collected. This mark
is not used by the device, but it is available to the user and
with it a significant change (for example the addition of a
sample or of another reagent) can be highlighted. The mark
is also shown in the table.

Select Stop to stop taking sample readings.

6.6.3

Analysis of time course data

After the Time Course data has been collected, the following
manipulations can be done on the graphic data:

The Time Course Program is complete, if

the instruments beeps after completion of the overall time,

the graph is mapped over the entire area,

the scaling of the x-axis is automatically adjusted,

the cursor functions in the navigation menu (right, next to the
graph) are activated.

6.6.3.1

Navigation of a time scan or a time scan analysis

After a time scan has been completed, the time and the
absorbance/transmittance data is displayed as a curve.

Where the cursor is positioned on the curve, the elapsed time up
to this point and the corresponding absorbance are highlighted.

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