Luminex xPONENT for MAGPIX 4.2 - Manual (Page 100)

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Settings Subtab
Protocols > Protocols > Settings
Click Create a New Protocol on the Protocols tab to open the Settings subtab.
Name and description boxes - Type the name and description in the appropriate box.
Acquisition Settings
• Volume - This is the volume the instrument aspirates into the system for analysis. Type the
desired sample volume in microliters. Use values from 20 to 200 µl. To avoid air intake,
add at least 25 µl to the sample well in addition to the sample size. The default value is 50
µl.
• XY heater - Select Enabled to enable the XY heater. In the box type the desired value in
degrees Celsius. The temperature range is 35° to 60° C.
CAUTION: Acquiring data before the heater has reached the proper
temperature can compromise test results.
• Plate name - The name assigned to the plate. You can select a different plate from the list.
• Sample wash - Select this option for assays without a final wash step prior to reading the
plate on the instrument. This automatically washes each sample within the instrument.
Final washes are required for proper analysis.
Analysis Settings - Use this section to set the analysis type, set the number of standards
and controls, select an external analysis program, and choose whether or not to analyze
results while acquiring samples.
Protocols Page
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