Luminex xPONENT for MAGPIX 4.2 - Manual (Page 91)

There are action buttons at the bottom of the page. Most of these buttons are not displayed
unless you are working with a saved protocol. While you are creating a protocol, only Cancel
and Next buttons are displayed. After saving a protocol or opening a saved protocol, the
following buttons are displayed:
• New Std/Ctrl
• Plate Layout
• Delete
• Import
• Export
• Edit
• View
Protocol Procedures
Creating an Allele Call Protocol
Protocols > Protocols > Create New Protocol
This protocol contains no standards or controls. An allele call protocol compares groups of
two, three, or four analytes to identify genotypes.
To create an allele call protocol:
1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol.
The Protocol Settings tab opens.
2. In the Name box, type the name of the protocol.
3. In the Description box, type a description of the protocol.
4. In the Version box, type the version of the protocol.
5. In the Manufacturer box, type the manufacturer information for the protocol.
6. Define the settings in the Acquisition Settings section.
NOTE: Final washes are required for proper analysis. If you are not
performing a final wash step on your plate before acquisition on the
MAGPIX instrument, enable Sample Wash here. This automatically
washes each sample.
7. Define settings in the Analysis Settings section, selecting Allele Call as the analysis
type.
8. Click Next. The Analytes tab opens.
9. Click the desired analyte (bead ID) in the numbered grid. Information about the analytes
is displayed in a list on the right side of the grid. For an allele call analysis, you must
select a group of two, three, or four analytes.
10. Click Group to group analytes for the allele call. The grouped analytes display in a list to
the right. Select more analytes and then click Group again if you want to add another
group to the analysis.
11. Click and type an analyte name in the Name column to the right of the analyte grid.
12. In the Count box, type the desired bead count for each analyte. Click Apply All.
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