Luminex xPONENT for MAGPIX 4.2 - Manual (Page 70)

You can direct the system to acquire samples in replicate regardless of batch type. For
qualitative batches, qualitative results for replicates are averaged and the reported
interpretation is determined from this replicate average.
Replicates in quantitative batches are based on a standard curve that is generated by either
the “Fit of all standards” or “Mean of replicates”. The default is “Fit of all standards”. Unknown
samples are calculated from the standard curve. Test results for replicate samples are
averaged to determine the reported quantitative result denoted as “AVG”.
You can analyze an acquired batch using the analysis features of Qualitative, Quantitative,
and Allele Call algorithms.
Qualitative Analysis - Determines results as either positive or negative, reactive or non-
reactive, and so on. The system is flexible in defining custom result ranges, such as negative,
low positive, and high positive.
Quantitative Analysis - Determines the sample concentrations from standard curves using
regression methods, such as 4P or 5P logistic curve fitting. There are two main assay types:
non-competitive and competitive. In a non-competitive assay, the slope of a concentration
versus Median Fluorescent Intensity (MFI) standard curve is a positive number. That is, low
concentrations result in low MFIs and high concentrations result in high MFIs. Conversely,
competitive assays generate a standard curve with a negative slope, the endpoints of which
are high MFI/low concentration on the left, and low MFI/high concentrations on the right.
Allele Call - Compares groups of analytes in order to determine genotype. Groups can
include two, three, or four analytes for comparison. Set the call rate to determine what ratio
you want to set as the limit for the analyte comparison.
Current Batch Tab
Results > Current Batch
Use this tab to view results, statistics, and log information related to the current batch, and to
perform statistical analysis on batch results. This tab offers real-time monitoring of batch
sampling during acquisition through a display of sample bead statistics and analytes, and dot
Results Page
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