Luminex xPONENT for MAGPIX 4.2 - Manual (Page 31)

CAUTION: This system contains electrical and mechanical components that,
if handled improperly, are potentially harmful. Adhere to standard
laboratory safety practices.
CAUTION: Protection provided by the equipment can be impaired or the
warranty voided if the Luminex system is used in a manner not
specified by Luminex documentation or Luminex Corporation.
Biological Samples
CAUTION: Human and animal samples may contain biohazardous
infectious agents. Where exposure to potentially biohazardous
material—including aerosol—exists, follow appropriate biosafety
procedures and use personal protective equipment such as
gloves, gowns, laboratory coats, face shields, or mask and eye
protection. Use ventilation devices. Observe all local, state, and
federal biohazard handling regulations when disposing of
biohazardous waste material.
Dilute concentrated biological samples, such as plasma or serum, at least 1:5 with reagents
as part of assay setup or as a final dilution step to reduce the chance of system clogs. If you
are running a MagPlex
® kit, follow the dilution instructions in the kit’s product insert.
Bead (Microsphere) Handling
MagPlex beads come in various configurations. To reduce foaming and precipitation, avoid
agitating the beads until you are ready to vortex and use them. Beads settle and must be
resuspended by vortexing before use. In addition:
• Multiple pipetting from the original container can affect bead concentrations.
• Protect MagPlex beads from light at all times to prevent photobleaching. Photobleaching
effects are cumulative. To maintain the integrity of the beads, minimize their exposure to
light during your development and manufacturing phases.
• Store MagPlex beads at 2°- 8°C.
NOTE: Refer to the product information sheet that accompanies your
MagPlex beads or for additional information.
Repetitive MagPlex Bead Measurements
In a MagPlex assay, the reporter signal is the result of the assay. Due to small bead size,
MagPlex bead suspension exhibits near solution-phase reaction kinetics. This means that
each set of beads used for a particular assay shows a statistically even distribution of
reporter molecules bound to the surface of each bead. The fluorescence signal of reporter
molecules bound to the surface of each bead set is measured and used to determine the
result of each assay in a multiplex. During data acquisition, numerous beads of each set are
analyzed and the median statistic is computed for that set by xPONENT. The more beads of
a set measured, the more confidence that can be given for that particular measurement.
Luminex recommends that you use R-Phycoerythrin as your reporter fluorophore.
If you are running a calibration and verification kit, follow the kit's product insert or use the
software protocol provided.
xPONENT for MAGPIX 4.2 Software User Manual
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