Plate layout subtab – Luminex xPONENT for MAGPIX 4.2 User Manual

Page 103

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6. If you selected Lum Qual in the Formulas list, edit the values for each range under

Negative, Low Positive, or High Positive. If you selected Adv Qual in the Formulas
list, edit Negative, Low Positive, Moderate Positive, or Strong Positive. Select the
check box in the Inclusive column to include the value in the range. If you clear the
check box, the value will be one unit higher than the low value, and one unit lower than
the high value.

7. Click Add Range if you want to add a new range. Type a Range Name, Low Value,

High Value, and check or clear the Inclusive check boxes.

8. If you want to delete a range, click a range to highlight it, and click Delete Range.

9. Check Mark as Intra-Well Normalization Bead if you want to use a normalization bead.

The normalization bead is a microsphere set that is included in the assay as an internal
control. It controls for sample variation and can be used to normalize data between
samples in a run. An analyte used as a normalization bead will appear blue in the analyte
grid.

10. Type a name for the new formula in the Formula Name box.

11. Click Save Formula. The new formula displays in the Formulas list.

12. Click Apply to All Analytes to apply the new formula to all analytes in the list, or click

OK to apply the new formula to the single analyte you first clicked.

Plate Layout Subtab

Protocols > Protocols > Plate Layout

xPONENT for MAGPIX 4.2 Software User Manual
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