Luminex xPONENT for MAGPIX 4.2 - Manual (Page 77)

• Replay - Opens the Select Replay Mode dialog box. This box enables you to use the data
stored in the run files from the initial acquisition to reprocess a batch, creating a new batch
output file. A batch can be reprocessed multiple times. When you replay or recalculate a
batch, you perform the same steps to create the batch as you did when you created the
batch the first time. This sequence varies based on whether or not you created a new
batch from a new protocol or a new batch from an existing protocol. The initial batch data
and output file always remain intact and unchanged. Each time you replay a batch, the
system handles it as if it is new data, and creates a separate batch entry and output file.
• Replay batch - Use to replay raw bead data files. The bead data files are replayed
using the gate, analyte, analysis settings, and plate layout that were selected in the new
or updated protocol. Settings such as bead type, volume, timeout, XY heater, and report
gain have no effect on the replayed results.
• Recalculate data - Reanalyze the batch results using only the batch MFI values. The
batch MFI values are recalculated using the analysis settings and plate layout selected
in the new recalculated batch or protocol. Settings such as bead type, volume, timeout,
XY heater, and report gain have no effect on the replayed results. Because only the MFI
values are reanalyzed, no data is displayed in the dot plot.
• OK - Saves your changes.
• Cancel - Cancels your changes and closes the box.
• Open - Opens the Results tab. Use this tab to view the saved batch results for the
selected batch. When you click Open, the buttons change:
• Save Image - Opens a Save As dialog box to save a screen capture.
• Formula - Opens the Change Analysis dialog box with a list of analytes used in the
batch.
xPONENT for MAGPIX 4.2 Software User Manual
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