Luminex xPONENT for MAGPIX 4.2 - Manual (Page 29)

• Remote Web Monitoring - Enables you to view alerts and system status using a
webpage.
• LIS - Enables the system to communicate with an external Laboratory Information System
(LIS) database. The LIS package enables you to export and import patient result data in
ASTM file format.
You must have an instrument control license to operate the instrument.
For more information about purchasing upgraded packages, or to obtain specific package
documentation, contact your vendor.
MAGPIX Technology
The MAGPIX system operates by using magnetic beads (microspheres) that are coated with
a reagent specific to a particular bioassay, enabling the capture and detection of specific
analytes from a sample. The sample mixture is aspirated by the sample probe and conveyed
via Drive Fluid into the camera chamber, where the beads are pulled down into a monolayer
by the magnet, immobilized, and imaged. Within the chamber, beads are exposed to a red
LED and a green LED, which excite both the internal dyes that identify each bead’s color
signature and the reporter fluorescence from the surface of the beads. The red LED is
responsible for classifying the beads. The CL1 and CL2 filters function to categorize the
beads based on color signature and place them properly on the bead map as well as throw
out any doublets that may exist. The green LED with the RP1 filter excites the reporter
fluorescence, which identifies the quantity of analyte captured for each bead region. The
beads are then flushed to the waste container, clearing room for the next sample.
Calibration is important to ensure that the optical system functions effectively and that
different Luminex MAGPIX systems report similar results. Calibrating the MAGPIX system
normalizes the settings for the classification channels (CL1 and CL2) and the reporter
channel (RP1). Use the Luminex MAGPIX Calibration Kit to accomplish this.
Following calibration, use the Luminex MAGPIX Performance Verification Kit to check all of
the optical channels in the system for correct calibration. It is essential to verify every time
you calibrate. If there is a problem with optical integrity or fluidics, MAGPIX may pass
calibration but fail performance verification. The Luminex MAGPIX Performance Verification
Kit includes reagents to verify the calibration and optical integrity for the Luminex MAGPIX
system as well as reagents to verify the fluidics channels using observations of bead count
and well-to-well carryover.
xPONENT for MAGPIX 4.2 Software User Manual
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