Setting up batches, Using the batches page – Luminex xPONENT for MAGPIX 4.2 User Manual

Page 43

Advertising
background image

Analytes - Displays when Create a New Batch from a new Protocol is clicked.

Plate Layout - Displays when Create a New Batch from an existing Protocol and

Create a New Batch from a new Protocol are clicked.

New MultiBatch - Displays when Create a New Multi-Batch is clicked.

NOTE: These tabs (except New MultiBatch) are sequential. You must

complete each screen in a specific order.

The Pending Batches list displays the name of the protocol used with the batch, the protocol
version, date, and status for each pending batch. The following buttons only appear if the
pending batches have data:

Single Step - Instructs the system to acquire one well and then pause. If Single Step is

activated during a batch, the batch pauses at the end of the current well. This ensures that
the system is working correctly before you run an entire batch.

Save Prtcl. - Saves protocol and/or assay information for a standard/control.

Plate Layout - Opens the Report dialog box, which includes the Batch Plate Layout

Report.

Import - Imports a batch not previously run in xPONENT 4.2 from a folder on the PC into

xPONENT.

Export - Exports the batch information in order to move it to another computer, make a

copy of the data, and then import it into xPONENT on another computer.

Delete - Deletes a batch.

Edit - Edits a batch.

Run - Runs a batch.

Setting Up Batches

Batches consist of protocols and samples for acquisition and can span more than one plate.
Protocols contain predefined commands that must be included in every batch acquisition.
You can group batches together as a multi-batch. Multi-batches can consist of any number of
batches that have been set up from different protocols and are processed consecutively.
Multi-batches cannot be run on multiple plates.

NOTE: When setting up a batch, if the number of samples exceeds the

number of wells in one microtiter plate, you can add additional plates
in the Add and Change Plate secondary window. Additional plates
are identified on the bottom of the plate image as Plate a of b, where
a is the plate number and b is the total number of plates.

Assay kit manufacturers may provide protocols in their kits which are distributed on a CD.
Protocols typically include assay standards, controls, and maintenance commands (such as
washes or primes to acquire along with samples). Assay reagents are included in assay kits.
You must provide information about these reagents, such as lot numbers and concentration
values for the standards and assay controls.

Using the Batches Page

xPONENT for MAGPIX 4.2 Software User Manual
30

Advertising