Luminex xPONENT for MAGPIX 4.2 - Manual (Page 123)

WARNING: When using multiple plates, use them in the proper order.
Failure to do so can result in inaccurate data and test results.
Commands - This section contains the following commands:
• Wash - Sends distilled water through the fluidic lines in the system. It pulls the fluid from
a well or from the reservoir and runs it completely through the system to the waste
receptacle.
• Alcohol Flush - Removes air bubbles from the sample tubing and the cuvette using
70% isopropanol or 70% ethanol. The alcohol flush takes about five minutes. Alcohol
Flush uses the Luminex XYP reservoir because only the reservoir can accommodate
the amount of fluid necessary to flush the instrument.
• Sanitize - Uses the off plate reagent area, because only the reservoir can accommodate
the amount of fluid necessary to sanitize the instrument. The sanitize command
performs a similar function as the alcohol flush command, but uses 10% to 20%
household bleach and water solution to decontaminate sample lines and the cuvette
after biohazard contact. Sanitize as part of your daily shutdown routine after biohazard
contact.
• Clean - Aspirates a cleaning reagent such as bleach or sodium hydroxide.
• Soak - Prevents salt crystals from forming in the probe due to air exposure. Soaking the
probe replaces Drive Fluid in the probe with water. You should perform the soak function
at the end of each day. The system uses at least 250 µL of distilled water.
• Rinse - Performs a rinse.
• Prime - Removes air bubbles from the systems fluidic pathways by drawing Drive Fluid
from the Drive Fluid container. You do not need to supply solution in a plate.
• CAL - Performs a calibration.
• VER - Performs a verification.
• Fluidics - Runs fluidics.
Click a command to add it to a new routine, to modify an existing routine, or to run the single
command. It will appear in the plate image to the right of the Commands. You can also
select a location first, which places the command where you choose. To change the location
of a command already on the plate, select it in the Command Sequence, and then click a
different well or reservoir in the plate image.
NOTE: When you modify an existing routine, by adding or clearing a
command, the routine name defaults to None in the Routine Name
drop-down menu and the Save As button appears. Remember to
save your new routine to make it available for future use.
Command buttons are located to the right of the Command Sequence box:
• Clear - Clears the highlighted command in the Command Sequence list.
• Clear All - Clears all commands in the Command Sequence list.
Different buttons appear at the bottom right of the window, depending on your selections in
the window:
xPONENT for MAGPIX 4.2 Software User Manual
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