Luminex xPONENT for MAGPIX 4.2 - Manual (Page 23)

1. Adjusting the Sample Probe Height
2. Revive After Storage (Luminex) Routine
3. Calibration/Verification
Adjusting the Sample Probe Height
Adjust the sample probe height to ensure that the probe drops far enough into the well to
acquire sample.
NOTE: Ensure that there is no liquid in the wells or reservoirs before
adjusting the sample probe height.
1. On the Home page, click Probe and Heater under Daily Activities. The Probe &
Heater tab opens.
2. Use well D6 (this is the center of a standard 96-well plate).
3. Ensure that the well location is selected on the plate image. A green pin marks the
selected well.
4. Based on the type of plate you are using, place alignment disks or an alignment sphere in
the well.
• For a standard 96-well plate - none
• For a Filter-bottom plate - two 5.08 mm disks
• For a Mylar-bottom plate - two 5.08 mm disks
• For a conical (v-shaped) plate - one sphere
5. Click Eject to eject the plate carrier.
6. Place the off plate reagent block on the plate carrier. Make sure it is well seated so that it
clips into place.
7. Place a strip well (provided with the Calibration and the Performance Verification kit) in
the off-plate reagent block.
8. In the Strip Wells section, click SD1.
9. Verify that the reservoir is empty.
10. In the Reservoir section, click well RB1.
11. Verify that the plate is not warped. Warped plates can lead to incorrect probe height
adjustment.
12. Place the plate on the plate carrier with well A1 positioned as indicated on the plate
carrier.
13. Click Retract to retract the plate carrier.
14. Type a name for the plate in the Plate Name box.
15. Click Auto Adjust Height. The probe automatically adjusts itself to the locations you
selected.
NOTE: The probe height is automatically set to 0.98 mm. The probe
automatically adjusts this distance from the bottom of the plate, or
calibration disks or spheres.
16. Click Eject to eject the plate holder. If you used alignment disks or spheres, remove them
from the plate.
xPONENT for MAGPIX 4.2 Software User Manual
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