Luminex xPONENT for MAGPIX 4.2 - Manual (Page 92)

13. To set a bead count and the units for a single analyte, click in the Units and Count
columns directly to the right of the analyte grid, and type a units value and bead count.
14. Click in the Call % column and type a value to set an individual analyte's call percentage.
15. Click Next. The Plate Layout tab opens.
• To add well commands, select the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you've
added and change the starting location on the plate. If you want to run in replicate,
change the Replicate Count to the appropriate number and the Grouping to your
preferred grouping method.
• Delete a command by clicking the well, and clicking Delete. The Delete Options
dialog box opens. Select Delete just the selected wells to delete a single well
command, or Delete all wells containing these samples to delete all wells with the
same command.
NOTE: The Post Batch routine runs after the batch is complete. This routine
is selected by default when the protocol or batch is set up. The
default Post Batch routine can be changed in the Batch Options
tab of the Admin page.
NOTE: For more information about the commands and settings on the Plate
Layout tab, see the Plate Layout Tab .
Creating a Quantitative Assay Protocol
The protocol must contain multiple standards. The standards are assigned lot value
information for each test. A standard curve is generated according to the lot values. Including
controls in the protocol is optional, but recommended for judging the acceptability of batch
results.
To create a quantitative assay protocol:
1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol.
The Settings tab opens.
2. In the Name box, type the name of the protocol.
3. Type a description in the Enter optional description here box.
4. In the Version box, type the version of the protocol.
5. In the Manufacturer box, type the manufacturer information for the protocol.
6. Define settings in the Acquisition Settings section.
7. Define settings in the Analysis Settings section, selecting Quantitative as the analysis
type. The protocol for a quantitative assay must contain multiple standards. Controls are
optional in a quantitative assay protocol.
8. Click Next. The Analytes tab opens.
9. Click the desired analytes (bead ID) in the numbered analyte grid. Information about the
analyte displays to the right side of the grid.
10. Click and type an analyte name in the Name column to the right of the analyte grid.
11. Click and type the desired unit of measurement in the Units box to the left of the Apply
All button.
12. Click and type the desired bead count for each analyte in the Count box. Click Apply All.
Protocols Page
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