Luminex xPONENT for MAGPIX 4.2 - Manual (Page 81)

• Statistic - To view a particular statistic for analytes in a batch, select one of the options on
the drop-down list. The statistical options displayed change according to the type of
analysis.
NOTE: Trimmed statistics (indicated by *) remove the lower and upper five
percent of the extreme statistic values, then use the remaining
values for the Mean, Standard Deviation, or %CV calculations. The
point of a trimmed statistic is that it removes outliers, ensuring that
the data is more representative of the population.
• Median (MFI) - The value (detected signal) that is in the middle of the microsphere
population when sorted by reporter value lowest to highest. The mediam value is much
less sensitive than the mean value to outliers and carryover.
• Test Result - The calculated analysis value for quantitative or qualitative assays derived
from standards with known values.
• Range - A semi-quantitative result for a particular numerical result falling between a
predefined set of values such as Normal or Negative.
• Count - The number of microspheres detected within the specified microsphere region.
Microspheres that are not inside the region on the dot plot are not included.
• Net MFI (Sample Well MFI - Background Well MFI)) - The NetMFI can be used to
eliminate the effect of background signal in an assay.
• Mean - Average of all values for the microspheres detected in a region.
• % CV of microspheres - The measure of relative dispersion within the distribution.
%CV = 100 x Std Dev / Mean
• Standard Deviation - For calculating sample variability or dispersion, Luminex uses the
standard deviation formula.
• Peak - The value that is equal to the largest number of data points within the distribution.
For example, in data set {1,2,2,3,3,3,4,5}, 3 is the peak because it occurs the most
frequently in the distribution list.
• Trimmed Count*
• Trimmed Mean*
• Trimmed%CV of microspheres*
• Trimmed Standard Deviation*
• Trimmed Peak*
• % CV of Replicates - The measure of relative dispersion within the distribution of results
for replicate samples.
%CV = 100 X Std Dev / Mean
• % Recovery - A measure of how accurately your observed results match your expected
results following regression analysis.
(Observed concentration) / (Expected concentration) x 100%
• Expected Result - The known or expected test result value for a standard or control.
• Control Range - Low - The lowest value for an assay control used to determine pass/
fail criteria for an assay.
xPONENT for MAGPIX 4.2 Software User Manual
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