Luminex xPONENT for MAGPIX 4.2 - Manual (Page 25)

3. Add 70% isopropanol or ethanol to reservoir RB1 on the off-plate reagent block as
indicated on the Cmds & Routines tab.
NOTE: The rinse reservoir (RD1) should be empty.
4. Click Retract.
5. Click Run.
After the Revive After Storage routine is complete, run the System Initialization routine.
System Initialization
xPONENT for MAGPIX contains a pre-defined routine to prepare the analyzer for data
acquisition. This section describes calibration and performance verification of the system.
Calibrator magnetic beads are used to normalize the settings for the reporter channel and
classification channels. Verification magnetic beads are used to verify calibration and optical
integrity of the system. Fluidics beads are used to assess well-to-well carryover.
If the system is not fully calibrated, a warning message opens.
Once calibrated, the values remain until you recalibrate. You can track system calibration and
verification results through the Calibration and Verification report. Target value information
for calibration and verification beads is available on the Luminex website at http://
www.luminexcorp.com/Support/index.htm.
Calibrate your system at least once a week using the Calibration/Verification button on the
Auto Maint tab of the Maintenance page. In addition, recalibrate the system if any of the
following things occur:
• The delta calibration temperature exceeds ± 5° C.
• You move the instrument.
• You experience sample acquisition problems.
• The instrument undergoes hardware maintenance, such as replacement of a part.
Verify the system daily using the Performance Verification button on the Auto Maint tab of
the Maintenance page. Refer to your assay kit instructions for additional calibration
frequency requirements.
Before you can calibrate the system, you must import MAGPIX calibrator and verification
bead lot information. Do this using the Lot Management tab of the Maintenance page. This
information is available on the CD that accompanies the Performance Verification Kit and
Calibration Kit, and is also available on the Luminex website at http://www.luminexcorp.com/
Support/index.htm.
xPONENT for MAGPIX 4.2 Software User Manual
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