Luminex xPONENT for MAGPIX 4.2 - Manual (Page 102)

• Fit of all Standards - The standard curve will be determined by using each individual
standard replicate when calculating the standard curve. For example, if you run duplicates
of a seven-point standard curve, the software will calculate the standard curve by using 14
points. Applies only to quantitative analysis.
• Mean of Replicates - The standard curve will be determined by averaging the individual
standard replicates when calculating the standard curve. For example, if you run duplicates
of a seven-point curve, the software calculates the standard curve by using 7 averaged
points. Applies only to quantitative analysis.
Cancel - Returns you to the main Batches tab.
Next - Click to advance to the Analytes tab.
Editing Quantitative Analysis Settings
To edit quantitative analysis settings:
1. Open the Protocols page, then open the Protocols tab. Select a protocol and click Edit.
2. From the Analytes tab, click an analyte’s Analysis field to open the Analysis Settings
dialog box.
3. From the Method list, click No Analysis, Cubic Spline, Linear Fit, Logistic 4P, or
Logistic 5P.
4. From the Weight list, click None or 1/Y ^2 .
NOTE: The Weight options are only available if the chosen Method is
Logistic 4P or Logistic 5P.
5. Check Mark as Intra-Well Normalization Bead if you want to use a normalization bead.
The normalization bead is a microsphere set that is included in the assay as an internal
control. It controls for sample variation and can be used to normalize data between
samples in a run.
6. Check Use Threshold Ranges if you want to use a range for the analysis.
7. Click Add Range to setup the threshold range.
8. Type a name for the range in the Range Name field.
9. Type low and high range values in the Low Value and High Value fields.
10. Select the check box in the Inclusive column to include the value in the range, or leave it
clear to make the range value one unit higher than the low value, and one unit lower than
the high value.
11. Highlight a range and click Delete Range to delete the range.
12. Click OK to apply the new settings to the analyte first clicked, or Apply to All Analytes to
apply them to all the analytes in the protocol.
Editing Qualitative Analysis Settings
1. Open the Protocols page, then open the Protocols tab. Select a protocol and click Edit.
2. On the Analytes tab, click an analyte's Analysis field on the right side of the window to
open the Analysis Settings dialog box.
3. From the Method list, click Luminex Qualitative or No Analysis.
4. From the Formulas list, click Lum Qual or Adv Qual.
5. Type a new name for the edited formula in the Formula Name box.
Protocols Page
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