Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 108)

Microplate Manager User Guide
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This report will be unavailable if you elected to ratio the results of a dual wavelength
reading in the Protocol.
In the Absorbance Report, the mean absorbance of all the blank wells in an assay is
subtracted from the raw absorbance value of each non-blank well in the same assay.
If no blank wells have been defined, the values in the Absorbance Report and the
Raw Data Report will be the same.
A negative value will be reported if the mean absorbance of the blank wells is greater
than the absorbance of the unknown well.
Absorbances are displayed only for standards, unknowns, and positive and negative
controls.
Ratio Report
This report is available for Endpoint and Multiple Plate Protocol data. With the Data
File open, select Ratio Report from the VIEW menu.
This report is only available if you elected to ratio the results of a dual wavelength
reading in the Protocol (see page 26).
In the Ratio Report, the mean ratio of all the blank wells in an assay is subtracted
from the raw ratio value of each non-blank well in the same assay. If no blank wells
have been defined, the ratios in the Ratio Report and the Raw Data Report will be the
same.
A negative ratio will be reported if the mean absorbance of the blank wells is greater
than the absorbance of the unknown well.
Velocity Report
This report is available for Kinetic Protocol data. With the Data File open, select
Velocity Report from the VIEW menu.