Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 58)

Microplate Manager User Guide
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Figure 36. Absorbance Delay dialog box.
In the Absorbance Delay dialog box, click on the Use Absorbance Delay checkbox
to enable this feature. Select the row and column number of the well whose
absorbance you want to monitor from the pull-down lists. In the Acquire when the
reading is section, specify an absorbance that is above or below a certain OD value.
Below will begin data collection when the measured absorbance is below the
specified OD, and Above will begin data collection when the absorbance is equal to
or greater than the specified OD.
Click on the OK button to implement your changes.
Ratio Delay, available for dual wavelength ratio measurements, delays data
collection until a well reaches a specified measurement/reference wavelength ratio.
Click on the Ratio Delay button, and enter a measurement/reference ratio in the
appropriate field.