Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 51)

Chapter 4. Protocols
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For cases where the absorbances decrease with time, the velocities will be negative.
In these cases, select the Negative Kinetics checkbox.
If the velocities are negative, then the velocity or maximum velocity axis of the
Standard Curve Report is constrained to linear unless the Negative Kinetics box is
checked (logarithms cannot be taken of negative values).
Multiple-Plate Protocol Features
A Multiple Plate Protocol performs single Endpoint readings on a series of plates and
saves those readings as separate Data Files. It can also export them in text format.
This Protocol may be used with or without a plate loader. With a plate loader, you
program the loader to load your plates in sequence. Without a plate loader, you will
be prompted to manually load and unload each plate in the sequence, up to the
number you specify in the Protocol.
To run a new Multiple Plate Protocol, select New Multiple Plate Protocol from the
FILE menu. This will open the Multiple Plate Protocol dialog box.