Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 92)

Microplate Manager User Guide
84
If more than one assay has been defined on your plate, click on the tabs at the top of
the window to switch between assays. Note that different assays can have different
curves, using different regression methods, etc.
Select the Show Labels checkbox to display labels for the individual standards on
the curve.
Select the Swap XY Axes checkbox to switch the X and Y axes of the curve. This
will affect the curve display only, not the calculated data.
To find the coordinates of a particular point in the graph, position your cursor on that
point. The coordinates will be displayed in the status bar at the bottom of the
Microplate Manager window. (The status bar display is toggled on and off by
selecting Status Bar under the VIEW menu).
Regression Types
In calculating the standard curve, you can select from among eight different
regression methods: Linear, Quadratic, Cubic, Four-Parameter Logistic, Five-
Parameter Logistic (Cook), Five-Parameter Logistic (Rodbard), Cubic Spline, and
Point to Point. To change the regression method, select from the Regression Type
pulldown list.
Figure 63. Selecting a regression method.