Bio-Rad Model 680 Microplate Reader Accessories User Manual

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Chapter 3. Getting Started

17

Scenario 1. Endpoint Assay with Previously Formatted
Template

You want to perform an Endpoint assay using a template layout that you have already
defined. From the FILE menu, you select New Endpoint Protocol. This opens the
Endpoint Protocol dialog box. You enter Mix Time, Initial Wait, and Incubator
settings for the reader.

Figure 10. Performing an Endpoint reading.

You have a Data File saved from a previous run with the template you want to use for
this plate, so you click on Pick Template... and choose that Data File. Now that
everything is set, you click on the Run button.

After the reading is complete, the raw data values pop up. The plate template is also
displayed. Since the template already contains the concentrations of the standards,
you go straight to the Unknown Concentration Report (select Unknown

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