Entering dilutions of unknowns – Bio-Rad Model 680 Microplate Reader Accessories User Manual
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Microplate Manager User Guide
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• Run at least three replicate wells for each dilution of standards and samples.
• Run an adequate number of sample dilutions to ensure that more than one
dilution lies in the middle of the curve. Running several dilutions will point out
the limitations of the curve-fitting methodologies.
Entering Dilutions of Unknowns
You can enter dilution factors (if any) for the unknown wells in a template. Select
Edit Unknown Dilutions... from the EDIT menu. This will open the Edit Unknowns
dialog box.
Figure 53. Edit Unknowns dialog box.
Each defined assay has a tab that is labeled with the name of the assay (default names
are Assay 1, Assay 2, etc.). Dilutions for each assay are entered separately on a form
under each tab. To enter dilutions for Assay 2, click on the Assay 2 tab.
Each unknown is listed in the column at the left. The default dilution is 1.