Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 26)

Microplate Manager User Guide
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Concentration Report from the VIEW menu). You select Print... from the FILE
menu to print this report.
You decide that you will be repeating this operation frequently, so you go back to the
Endpoint Protocol dialog box. The Reports... button opens a dialog box in which
you specify the reports to view and/or print after each reading. Select the Unknown
Concentration Report for printing, and click on OK. You select Save Protocol from
the FILE menu and enter a file name to store this Protocol on disk.
The next day, you want to repeat the Protocol. Select the Protocol File name from the
Open dialog box, place your plate in the reader, click on the Run button, and wait at
the printer for a hard copy of the new Unknown Concentration Report.
Scenario 2. Kinetic Assay
You want to perform a new Kinetic assay. From the FILE menu, you select New
Kinetic Protocol. The Kinetic Protocol dialog box opens.
For this assay, you would like to make five readings at 22-second intervals followed
by 20 readings at one-minute intervals. You want to shake the plate for one second
before each of the first five readings.
To accomplish this, you define two reading sets. The first set has 5 repetitions, a
1-second mix time, and a 22-second interval (note that the interval you specify
includes the mix time). The second set has 20 repetitions, 0 seconds mix time, and a
60-second interval.
Figure 11. Defining the Reading Sets.
Interval includes
mix time