Bio-Rad Model 680 Microplate Reader Accessories - Manual (Page 75)

Chapter 5. Templates
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If your well numbering is out of sequence and you want to correct it, select
Resequence Well Names from the FORMAT menu. The wells will be renumbered in
the correct order. This is a cosmetic change, and will not affect the analysis in any
way.
Defining Blanks and Controls
Blank wells are used to measure background noise for accurate absorbance
calculation. Positive control wells contain samples that define the upper limit of your
data range. Negative control wells contain samples that define the lower limit of your
data range.
You should define any assays before specifying well types.
To format blank wells, click on the Format as Blanks button, then click or drag on
the blank wells in the template.
To format positive control wells, click on the Format as Positive Controls button,
then click or drag on the positive control wells.
To format negative control wells, click on the Format as Negative Controls button,
then click or drag on the negative control wells.
To change a formatted well back to undefined, click on the Format as Undefined
button, then click or drag on the defined wells.
If you have defined multiple blank, positive control, or negative control wells in a
template, all wells of the same type are treated like a replicate group (see page 68),
and a mean value, standard deviation, and coefficient of variation are calculated for
the group.
To change the type of a well or group of wells, simply select the appropriate well
type tool and overwrite them, as if you were defining the wells for the first time.